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IVF – In Vitro Fertilization

In Vitro Fertilization (IVF) Treatment

The process of IVF (In Vitro Fertilization) is a laboratory procedure where the egg is fertilized with the sperm in a laboratory dish and is implanted into the woman’s uterus.

Intra Cytoplasmic Sperm Injection (ICSI)

Intra Cytoplasmic Sperm Injection (ICSI) is an assisted reproductive technique where in a single sperm is injected into the cytoplasm of the oocyte with the help of special micro-manipulators.

IVF Process

Initial Consultation with Oasis Fertility Specialist


What is the IVF treatment procedure?

The initial steps for both IVF and ICSI are similar. Both treatments differ in the insemination methodology post the egg collection step.

Step 1: Pre-investigation to assess feasibility

Pre Treatment Investigations

Before subjecting a couple to the IVF procedure they are advised to undergo certain investigations to check the feasibility for the procedure.

The female partner is asked to undergo certain hormonal blood tests and the male partner is asked to undergo basic semen analysis. Both the partners are subjected to certain common tests like blood grouping, HIV, Hepatitis B & C, and blood tests for sexually transmitted diseases as part of the IVF process.

Step 2: Long or Short protocol

Following the pre-assessment, the next step could either be a long protocol or a short protocol. The doctor takes a call on what treatment needs to be followed.

Long Protocol

The duration of the treatment is about one month and can be divided into two phases mainly:

  1. Suppression Phase: The female partner is required to come to the hospital for a daily subcutaneous injection usually from day 22 of the cycle (day 1 is the first day of the menstrual period) for about 15 days.
  2. Stimulation Phase: After about 15 days other set of injection/s is/are added which stimulates the ovaries to produce eggs. These injections are administered by daily injection subcutaneously (sc) or intramuscularly (im) (into the buttock muscle).The daily dose may be increased or decreased depending on how well the ovaries are responding. Stimulation continues until the eggs are mature. This takes approximately 10 – 14 days.

Short Protocol

This usually takes about 15-18 days where injections and medications are administered to the female partner on an everyday basis. This continues until the eggs are mature.

Note: The timing of events during the IVF process is particularly crucial to the success of the pregnancies. Patients are asked to attend OASIS for an ultrasound scan of the ovaries during the treatment cycle which is usually after Day 5.

Step 3: Egg Collection

Based on the doctor’s assessment, a date is fixed for egg collection and the female partner’s eggs are removed under vaginal ultrasound guidance. There are no cuts on the abdomen.

Step 4: Semen Preparation and Embryo Transfer

The male partner’s semen is taken and IVF (where eggs are just mixed with sperm) or ICSI (where single sperm is injected into the egg) is carried out in the laboratory. This is then inseminated into the female partner’s uterus.

How are the procedures done?

Egg Collection:

Egg collection is a day care procedure which requires the patient to get admitted to the hospital for half a day. She would be evaluated by anesthesiologist a day or two prior to the egg collection. The eggs collected under vaginal ultrasound guidance are placed in an incubator. Generally, most of the follicles will carry a mature, healthy egg. However, not all follicles may yield an egg, or some may be immature or deteriorating and are unlikely to fertilize normally. It’s very rare that no eggs are found. If the treatment cannot proceed because no eggs can be collected or the egg quality is poor, the doctor will discuss the probable causes of this with you and recommend an appropriate course of action.

Embryo Transfer:

The procedure is carried out by inserting a speculum into the vagina and cleaning the cervix. The patient may feel one cramp as an outer catheter is placed through the cervix into the lower segment of the uterus. A fine plastic catheter, into which the Embryologist has transferred the embryos, is then placed through the outer transfer catheter and advanced near the top of the uterus. The sonographer will visualize the lining of the uterus and guide the physician in the placement of the catheter. Once the placement is correct, the embryos will be expelled from the catheter and inserted into the uterus.

Associated Treatments: In Vitro Maturation of Oocytes

For more information regarding In Vitro Fertilization (IVF) or Intra Cytoplasmic Sperm Injection (ICSI), please visit the IVF FAQ page.


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